General Information：
Human Long Arg3 Insulin-like Growth Factor-I (Long-R3-IGF-I) is an 83 amino acid analog of human IGF-I comprising the complete human IGF-I sequence with the substitution of an Arg for the Glu at position 3 (hence R3), and a 13 amino acid extension peptide at the N-terminus. Scientists have engineered this analog with the express purpose of increasing biological activity. Human Long-R3-IGF-I is significantly more potent than human IGF-I in vitro. The enhanced potency is due to the markedly decreased binding of human Long-R3-IGF-I to IGF binding proteins which normally inhibit the biological actions of IGFs.
Expression Host: E. coli.
Molecular Mass:
The recombinant human Long-R3-IGF-I has a calculated molecular mass of 9111Da.
Formulation:
Lyophilized from sterile PBS , pH 7.2, and 5 % mannitol are added as protectants before lyophilization.
Quality Control
Purity: as determined by the following methods:
(a) > 95%, RP-HPLC analysis.
(b) >98%, SDS-PAGE analysis.
Bio-activity:
The ED50 of recombinant human Long R3 IGF-I, calculated by the dose-dependant proliferation of murine BALBC 3T3 cells, is less than 1.0ng/ml, corresponding to a specific activity of 1.0×106 IU/mg.
Endotoxin:
﹤1.0 EU per mg as determined by the LAL method.
Product specification：
100μg/vial；500μg/vial；1mg/vial.
Store at：
2-8oC for two years or -20oC for long term storage. Avoid repeated freeze and thaw